高原鼠兔低氧诱导因子-1α的初步研究 |
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出 版 社:兽类学报23(3):273-276 |
发表时间:2003 |
台 站:
海北高寒草甸生态系统定位研究站
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作 者:赵同标 赵新全 常智杰 朱姗姗 赵伟 孙平 徐世晓 |
点 击 率:4926 |
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关 键 字:关键词:低氧诱导因子-1α;高原鼠兔;RT-PCR;青藏高原 |
关 键 字(英文):Keywords:Plateaupika(Ochotonacurzoniae);HIF 1α;RT PCR;Qinghai TibetPlateau |
摘 要(英文):Abstract:Hypoxia inducedfactor 1playsakeyroleduringthecellhypoxiatransduction Hypoxiainducedfactor 1α(HIF 1α)isafunctionalsubunitofhypoxia inducedfactor 1 Plateaupika(Ochotonacurzoniae),whichisaQinghai Tibetplateaunativeanimallivedabove3000m,hashighratioofoxygenutilizationtoadapttoplateauhypoxiaenvironments Onefragmentofthecodingre gionofcDNAsequenceofplateaupikaHIF 1αwasobtainedbyRT PCRtechniqueusingadegeneracyPCRprimerbasedonprevi ouslyreportedcDNAsequenceinhuman,cattle,housemouseandNorwayratHIF 1αgene ItwasdirectlyinsertedintothevectorpMD18 T DNAsequencingprovedthatitwashighlyhomologywithhuman(91%),cattle(91%),housemouse(89%)andNor wayrat(89%)HIF 1αgene ThestudyprovidesbasicimportantinformationfortheHIF 1αcDNAwholesequencecloningofpla teaupikaanditsfunctionalstudy |
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