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研究报告目录 |
The effect of Iron and H2O2 on plasma membrane of wheat roots studied by Fourier Transform Infrared Spectroscopy |
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| 出 版 社：Cell research |
| 发表时间：2006 |
| 台 站：
沙坡头沙漠试验研究站
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| 作 者：赵昕 |
| 点 击 率：4285 |
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| 摘 要：We studied the alterations in structure and function within the plant plasma membrane (PM) caused by treatment with H2O2, FeSO4, and FeCl3 using attenuated total reflectance infrared (ATR-IR) and fluorescence spectroscopic techniques. It was observed that the 200 μM H2O2, FeSO4, and FeCl3 treated PM showed a decline in H+-ATPase activity and reduced membrane fluidity. Computer-aided analyses the FTIR spectrum indicated that the content of the α-helices decreased and βsheet increased in the secondary structures of proteins after exposure to the oxidants 200 μM H2O2, FeSo4, and FeCl3. The number of P=O and C=C bonds in the lipids of the membrane rapidly declined. These were the main reasons for the decline in H+-ATPase activity and the reduced fluidity of the PM. |
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