艳婀珍蝶取食对薇甘菊叶片生理指标的影响 |
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| 出 版 社：生态学报 |
| 发表时间：2006 |
| 台 站：
鼎湖山森林生态系统定位研究站
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| 作 者：张玲玲，韩诗畴，李志刚，刘楠，李丽英，罗莉芬，彭统序，刘文惠 |
| 点 击 率：3674 |
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| 关 键 字：薇甘菊；艳婀珍蝶；保护酶；总酚；有机自由基 |
| 摘 要：艳婀珍蝶取食后，对薇甘菊叶片的超氧物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、多酚氧化酶(PPO )的活性，总酚含量，有机自由基(DPPH·)清除能力进行分析。结果表明，取食后3h内所测各量即发生变化，但与对照差异不显著。取食4d中，叶片的SOD、PO D活性总体上分别高于对照，CAT活性总体上低于对照，说明在艳婀珍蝶胁迫过程中，SOD和POD所起的作用比CAT大。取食叶SOD、POD活性均在48h时达到最大值，之后下降；CAT活性在24h时达到最大值，之后也迅速下降。取食叶PPO 活性动态变化程度较大，表现出3个峰值，分别为对照的1.83，1.92倍和2.17倍；总酚含量表现为先上升后下降的趋势；对DPPH．的清除力一直显著性甚至极显著性低于对照。取食叶的SOD与CAT和POD均呈正相关性，且相关性大于对照；PPO 与总酚含量在取食叶与对照中也均呈弱正相关性。实验结果表明，薇甘菊的保护酶对艳婀珍蝶胁迫的应激效应是短暂而有限的，艳婀珍蝶的取食破坏了薇甘菊叶片功能，较大程度的干扰了薇甘菊保护酶系统的防御代谢，薇甘菊的总抗氧化能力降低。薇甘菊也不能通过改变酚类物质含量来抵御艳婀珍蝶的取食，艳婀珍蝶取食对薇甘菊有较明显的控制作用。 |
| 关 键 字(英文)：Mikania micrantha；Actinote thalia pyrrha；protective enzyme；total phenols；organic free radical |
| 摘 要(英文)：Mikania micrantha is a dangerous exotic weed that is now widely distributed in Guangdong，china．The lepidopteran
defoliator Actinote thalia Py a (Fabricius)，introduced from the Indonesian Oil Palm Institute，is a potential biological control
factor for M．micrantha．The change in activities of supemxide dismutase(SOD)，eatalase(CAT)，peroxidase(POD)，
polyphenoldoxidase(PPO)，the content of total phenolies and the capacity of scavenging 1，1一diphenyl一2一pierylhydazyl(DPPH．)
free radical in M ．micrantha leaves were analyzed after infestation by the larvae of A．thalia pyrrha．Th e results showed that a
slight change of all the indexes in damaged leaves appeared within 3 h．After four days of A． thalia Py a infestation，the
activities of SOD and POD in damaged leaves were higher than the eontml，but the activity of CAT was lower than the control
indicating that the functions of SOD and POD were impeded more than CAT．Th e activities of SOD and POD reached their hightest
value after 48h of A． thalia pyrha feeding，then decreased，while the highest activity of CAT occurred after 24h before
decreasing．Th e activity of PPO fluctuated greatly，its three peak values were 1．83，1．92 and 2．17 times that of the contro1．Total
phenolics content increased at first then decreased after 72h of A ．thalia pyrha feeding．The capacity of scavenging DPPH．was significantly lower than that of the control(P<0．05)．SOD activity was positivity correlated with CAT and POD activities for beth
damaged and normal leaves，however the value of correlation coeficiency in damaged leaves was higher than in normal leaves．The
content of total phenolics also showed a weak positive correlation with PP0 activity．It is suggested that the protective respo nse of
M ． micrantha to A． thalia py~ha infestation was short and limited， but resulted in reduced function of leaves，disturbed
metabolism in the protective enzyme system，and decreased antioxidative capacity． M ． micrantha was not able to resist the
feeding stress by altering content of total phenolics． |
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 艳婀珍蝶取食对薇甘菊叶片生理指标的影响国_2006 生态学报 张玲玲.pdf |
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