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Kinetics of alkaline phosphatase in lake sediment associated with
cage culture of Oreochromis niloticus
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出 版 社: Aquaculture [Aquaculture].  
发表时间:2001  
台  站: 东湖湖泊生态系统试验站  
作  者:Yiyong, Z; Jianqiu, L; Yongqing, F; Min, Z  
点 击 率:2351
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关 键 字:null  
摘  要:null  
关 键 字(英文):null  
摘  要(英文): Variations in kinetics of alkaline phosphatase occurring in
different sites of sediment associated with cage culture of
Oreochromis niloticus in a shallow Chinese freshwater lake (Lake
Donghu) were described. In addition, the kinetic parameters of
each 2.5-cm stratum in the sediment from the surface down to 37.5
cm were analyzed. Horizontally, the V sub(max) values of alkaline
phosphatase in surface sediments increased markedly at sites
immediately under and adjacent to the cage that would be subjected
to the deposition of fish feces. Peak V sub(max) values in the top
5 cm of the sediment under the cage were also observed relative to
their deeper control. After a treatment where the fish feces were
added over 12 days, the sediment in deeper layer exhibited a
significantly higher V sub(max) value, thereby corroborating the
relationship between V sub(max) values of alkaline phosphatase and
fish feces in sediments. The fish feces exhibited a remarkable
alkaline phosphatase activity (APA). Thus, it is indeed a source
of the enzyme. Effects of the fish feces were dose- and
time-dependent. The V sub(max) values in sediments were always
stimulated, but the K sub(m) values showed much more variability.
 
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